The Prairie Swept Field Confocal (SFC) Imaging System

A compact confocal system designed for high speed or high-resolution camera imaging through user-selected choice of supavideo rate, high frequency slit scanning or variable pinhole linear array scanning. The SFC is designed to interface with standard microscopes and CCD cameras.

The Prairie SFC System with Aurora Laser Launch includes:

• The Swept Field Confocal (SFC) Scanner with automated selection of sli 3 slit and 4 pinhole array aperture settings, scan control electronics and C-mount camera port for 1/2" CCD camera.
• Motorized 6-Position Filter Wheel for the SFC which holds standard 1" 25.4 mm) filters.
• High Performance Imaging Workstation PrairieView Software Package for the SFC.
• PrairieView provides control of scanning and image acquisition for optimization of data collection (includes control of image size, scan rate, camera settings, laser wavelength and power levels).
• 16 Bit, 10 MHz Digital Monochrome Camera: Includes charge multiplication gain capability. Also with advanced electronics provide EM Gain calibration with EM Gain and bias.
• Custom Modified BX51WIF Research Microscope including:
  • Nosepiece attachment arm for BX51WIF frame
  • Halogen Lamp with handswitch for intensity control
  • LSM-SWTR2-BX; Trinoc, Observation Tube
  • WI-SRE3 Swing out nosepiece
  • WI-OBCD; Long WD single oblique condenser, IR compatible
  • U-ACC: Achromatic/Aplanatic Condenser. N.A. 1.4 oil
  • BX-RFA; Fluorescence Illuminator
  • Fluorescence light source; X-Cite 120 for BX2 with iris and 3m LLG and epi-fluoresence cubes
  • Water Immersion M Plan Fluorite 10x Objective (UMPLFL10XW)
  • 40X Long Working Distance Water Immersion objective (LUMPLFL40XW/IR-2)
  • 60X Long Working Distance Water Immersion objective (LUMPLFL60X W/IR-2)
  • Prairie Z-Axis (focus) control automation for BX51WI (direct coupled micro-steepng motor).

Automated Specimen Stage for use with Prairie-Approved Microscope – Moving Platform with manual Z height adjustment range of ~ 2 inches.
Designed for electrophysiology experiments. Large platform stage on X-Y cross roller bearings to move attached electrodes (not included) and specimen in X and Y with respect to a fixed microscope. Height is adjustable over approximately 1.5 inches. Includes Prairie X-Y automation controller.

Aurora Laser to Fiber Launch with AOTF [ λ= ~488, ~561, ~643nm]
A laser launch to provide fiber optic delivery of selected laser lines via FC connector with Acoustic Optic Tunable Filter (AOTF) tuned to control included laser wavelengths.

System includes an Acoustic Optic Tunable Filter (AOTF) tuned to control included laser wavelengths, Laser launch optical plate with mechanical safety switches, and dichroics and optics for combining laser lines to AOTF. System also includes the following solid state lasers:
~488 nm Solid State Laser. ~50mW output, polarized
~561 nm Solid State Laser. ~50mW output, polarized
~643 nm Solid State Laser. ~40mW output, polarized SFC
System Installation and Training

The price for the system described above is approximately $200,000.00 (USD). Choice of microscope base, objectives, filters, lasers and other options may affect this price.

Please contact Prairie for more information.