A galvanometer-driven scan mirror serves as computer controlled mode switch element, which can direct beams from a variety of lasers into the microscope, where they illuminate the specimen. The SGS mini-scanner integrates the most refined and versatile technology used in high-resolution fluorescence imaging.
The AOD (Acousto-Optical Deflector) based option is a high speed scanning optics set that enables video rate two-photon imaging up to 1kHz at 34x34 pixel image size. Our novel design allows the user to switch between AOD mode and the conventional galvanometer-based mode, allowing the user to select the image speed as required by the experiment.
C. elegans embryo expressing B-tubulin GFP
Image courtesy of Koen Verbrugghe and Chris Malone, Laboratory of Molecular Biology, University of Wisconsin-Madison, Madison, WI
Time-lapse recordings of tubulin GFP C. elegans embryos during mitosis.
One image was acquired every second with a 100x Super Fluor lens using the SFC.
Image courtesy of Kevin Eliceiri and Koen Verbrugghe, LOCI, University of Wisconsin-Madison, Madison, WI.