Visible Light Photoactivation Module
Our Photoactivation Module couples visible laser light into the scan head such that it is collimated and aligned for point uncaging. Potential applications include highly accurate point (or spot) activation of photoactive and photoswitchable molecules such as Channelrhodopsin and Halorhodopsin.
Features:
- Single-mode fiber input
- Spot mode sizing capability with adjustable iris
- Variable spot size (5-25 μm spot) and diffraction limited point
- Pointing/positioning through use of second set of galvos
- Can take advantage of full visible light spectrum (400-700 nm)
DATA SPOTLIGHT
C. elegans embryo expressing B-tubulin GFP
Image courtesy of Koen Verbrugghe and Chris Malone, Laboratory of Molecular Biology, University of Wisconsin-Madison, Madison, WI
Time-lapse recordings of tubulin GFP C. elegans embryos during mitosis.
One image was acquired every second with a 100x Super Fluor lens using the SFC.
Image courtesy of Kevin Eliceiri and Koen Verbrugghe, LOCI, University of Wisconsin-Madison, Madison, WI.
