Single-Path Photolysis Head
The Single Path Photolysis Head is designed to deliver a programmable point or points of UV energy to a point within the field of view of the microscope objective lens. A moving optical aiming indicator shows the operator exactly where the UV energy will be directed without exposing the specimen to any additional light prior to photolysis. Easy-to-use controls allow precise positioning of the UV spot. The UV energy may be directly or remotely triggered. A fiber optic laser launch is required to deliver the UV energy into the Single Path Photolysis Head.
Optics
- Beam-combining optics
- UV-transmiting mirrors and optical surfaces
- Fiber launch to FC connector
Optical Fibers
- One UV transmitting fiber (8 foot length)
- Choice of fibers for selection of spot size
Photolysis Control via BNC input triggered by TTL pulse
- Control unit for UV X and Y positioning
- On/off switch for the aiming indicator light
DATA SPOTLIGHT
C. elegans embryo expressing B-tubulin GFP
Image courtesy of Koen Verbrugghe and Chris Malone, Laboratory of Molecular Biology, University of Wisconsin-Madison, Madison, WI
Time-lapse recordings of tubulin GFP C. elegans embryos during mitosis.
One image was acquired every second with a 100x Super Fluor lens using the SFC.
Image courtesy of Kevin Eliceiri and Koen Verbrugghe, LOCI, University of Wisconsin-Madison, Madison, WI.
