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Ultima In Vivo Multiphoton Microscopy System

Ultima IV with easy acces to objective

The Prairie Ultima In Vivo (IV) is specifically designed for the unique needs of the in vivo researcher. Its remarkable design provides 8-13 inches (20-33cm) of clearance under the objective lens for in vivo imaging of medium-sized organisms. Mounted on a post that can be either fixed directly to the table or mounted to a motor-driven X-Y stage, the user can choose to move either the specimen or the microscope. A built-in epifluorescence illuminator arm allows normal fluorescence imaging through the microscope eyepieces in order to locate the area of interest for study with two-photon imaging.

Options and Features

  • Modular design allows for flexible system configuration and add-ons
  • Highly customizable laser delivery optics; easy to share lasers with multiple systems
  • Imaging and optional uncaging galvanometer set
  • Fixed or X-Y translating base
  • Variety of detector options and configurations available
    • Dual or 4-channel non-descanned upper detectors
    • Dual channel non-descanned sub-stage detectors
    • Transmitted light Dodt gradient contrast detectors
  • Prairie View scan control and image collection software
  • TriggerSync software allows for uncaging and functional mapping experiments
  • Master/slave I/O triggers to start acquisition with a stimulus
  • High-performance PC workstation with flat panel display

PDF document Download the Ultima IV Specification Sheet (PDF)

Contact Prairie for more information or check our Frequently Asked Questions

DATA SPOTLIGHT

Real-time Calcium Wave Video

Real-time calcium wave in cardiac muscle.
Video Courtesy of Sarah Crowe and Graham Ellis-Davies, Mount Sinai School of Medicine.

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Mouse Lymph Node Video

3-D image of mouse lymph node
Image courtesy of Katalin Mikecz, M.D., Ph.D., Department of Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois.

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SFC

Z-stack of adult rat hippocampal CA1 pyramidal neuron in acute brain slice showing proximal dendritic arborization.
Image courtesy of Attilia Losonczy, M.D., Ph.D., Columbia University and Jeffrey C. Magee, HHMI-Janelia Farm

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