The Ultima In-Vivo (IV) Multiphoton Microscopy System
The Prairie Ultima IV is not built on a “standard” microscope. Its unique design provides 8-13 inches (20-33cm) of clearance under the objective lens for in-vivo studies of medium-sized organisms. Mounted on a post that can be either fixed directly to the table or mounted to a motor driven X-Y stage, the user can choose to move either the specimen or the microscope. An epifluorescence illuminator arm is included in the design. This allows the scientist to use normal fluorescence imaging through the microscope eyepieces in order to locate the area of interest for study with two-photon imaging.
Ultima IV Options and Features
- Open design allows for flexible system configuration and add-ons
- Highly customizable laser delivery optics; easy to share lasers with multiple systems
- Imaging and optional uncaging galvanometer set
- Fixed or X-Y translating base
- Variety of detector options and configurations available:
- Dual or 4-channel non-descanned upper detectors
- Dual channel non-descanned sub-stage detectors
- Transmitted light Dodt gradient contrast detectors
- Prairie View scan control and image collection software
- TriggerSync software allows for uncaging and functional mapping experiments
- Master/slave I/O triggers to start acquisition with a stimulus
- High Performance PC computer workstation with flat panel display
DATA SPOTLIGHT
3-D model of z-series from 5 month-old female H-Line mouse expressing YFP.
Image Courtesy of Sarah Crowe and Graham Ellis-Davies, Drexel University College of Medicine.
3-D image of mouse lymph node
Image courtesy of Katalin Mikecz, M.D., Ph.D., Department of Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois.
Z-stack of adult rat hippocampal CA1 pyramidal neuron in acute brain slice showing proximal dendritic arborization.
Image courtesy of Attilia Losonczy, M.D., Ph.D., Columbia University and Jeffrey C. Magee, HHMI-Janelia Farm