Two-Photon Acousto-Optic Deflector (AOD)
As an extension to the Ultima system, Prairie offers video-rate scanning with an Acousto-Optic Deflector (AOD).
AOD high-speed scanning is capable of 512x512 pixel imaging at 25fps and 32x32 pixel imaging at > 1 kHz, making this mode ideal for capturing momentary phenomena such as dendritic spikes.
The high-speed option is designed for specificity at a single excitation wavelength, determined at the time of installation. AOD is used for the X-axis scan and the standard galvanometer for the Y-scan. Switching between galvo and AOD imaging modes is software-controlled with Prairie View.
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DATA SPOTLIGHT
Real-time calcium wave in cardiac muscle.
Video Courtesy of Sarah Crowe and Graham Ellis-Davies, Mount Sinai School of Medicine.
3-D image of mouse lymph node
Image courtesy of Katalin Mikecz, M.D., Ph.D., Department of Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois.
Z-stack of adult rat hippocampal CA1 pyramidal neuron in acute brain slice showing proximal dendritic arborization.
Image courtesy of Attilia Losonczy, M.D., Ph.D., Columbia University and Jeffrey C. Magee, HHMI-Janelia Farm