Two-Photon AOD
High Speed Scanning with an Acousto-Optic Deflector (AOD) Prairie offers a high speed scanning options that may be added to Ultima system. This uses AOD for the X-axis scan and the standard galvanometer for the Y-scan.
AOD high speed scanning is capable of 521x512 pixel imaging at 25fps and 32x32 pixel imaging at > 1 kHz.
The high speed scanning option in no way detracts from the standard dual galvanometer method.
Switching between galvo and AOD imaging modes is software-controlled.
The high speed option is designed for specificity at a single excitation wavelength, determined at the tome of installation.
DATA SPOTLIGHT
3-D model of z-series from 5 month-old female H-Line mouse expressing YFP.
Image Courtesy of Sarah Crowe and Graham Ellis-Davies, Drexel University College of Medicine.
3-D image of mouse lymph node
Image courtesy of Katalin Mikecz, M.D., Ph.D., Department of Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois.
Z-stack of adult rat hippocampal CA1 pyramidal neuron in acute brain slice showing proximal dendritic arborization.
Image courtesy of Attilia Losonczy, M.D., Ph.D., Columbia University and Jeffrey C. Magee, HHMI-Janelia Farm