Flattened 3D Rendering looking up from bottom of stack
Image acquired on Prairie Ultima IV imaging system with Hamamatsu GaAsP detectors using an Olympus 20x/0.95 NA objective and a Coherent Chameleon ultrafast laser set at 925 nm. Image step size 5 microns, frame size 600 microns square, 512x512 pixels, 4 us/pixel dwell time.

3-D reconstruction of z-series from 5 month-old female H-Line mouse expressing YFP
Image Courtesy of Sarah Crowe and Graham Ellis-Davies, Drexel University College of Medicine.
Neuromuscular junction from the ceratomandibularis muscle of a lizard
The neuromuscular junction stained with primary mouse IgG antibodies raised against human cannabinoid (CB1) receptors, then with secondary goat anti-mouse antibodies complexed to Fluorescein. The CB1 receptors (GREEN) are clustered around the periphery of the motor nerve terminals and boutons (RED).
Courtesy of Dr. Clark Lindgren, Grinnell College, Grinnell, IA
Purkinje cell in a cerebellar slice
Image courtesy of Tiago Branco, Kazuo Kitamura, Michael Häusser, Wolfson Institute for Biomedical Research, University College London, London
In vivo multiphoton image in GFP mouse somatosensory cortex
Image courtesy of Dr Rafael Czajkowski, Silva Lab, Department of Neurobiology, UCLA
Striatal medium spiny neuron(MSN) from atrasngenic mouse (BAC D2)
Image Courtesy Dr. Michelle Day, Department of Physiology, Northwestern University Medical School, Chicago, IL
CHO K1 (Chinese Hamster Ovary) cells expressing paxillin-GFP
Paxillin is an adaptor molecule found in focal adhesions (large bright structures within the images). The pictures were taken on an Olympus IX70 with a QImaging Retiga Exi camera and Prairie laser launch. The wide field image is with the laser going straight through the sample and the TIRF image is with the laser tuned to the TIRF mode.
Image courtesy Claire M. Brown, PhD., University of Virginia, Charlottesville, VA (now at McGill University, Montreal, Canada)
3-D Image of Mouse Lymph Node
Credit: Image Courtesy of Katalin Mikecz, M.D., Ph.D.Department of Orthopedic Surgery, Rush University Medical Center, Chicago, Illinois
Rabbit Retina
Obtained on the Prairie SFC.
Courtesy of the University of Minnesota
C. elegans embryo expressing B-tubulin GFP
Image courtesy of Koen Verbrugghe and Chris Malone, Laboratory of Molecular Biology, University of Wisconsin-Madison, Madison, WI
Time-lapse recordings of tubulin GFP C. elegans embryos during mitosis.
One image was acquired every second with a 100x Super Fluor lens using the SFC.
Image courtesy of Kevin Eliceiri and Koen Verbrugghe, LOCI, University of Wisconsin-Madison, Madison, WI.