The Ultima is designed to include two sets of conventional galvanometer based scanners, the standard imaging set and the optional uncaging set, for simultaneous imaging and uncaging using two lasers.

The AOD based option is a high speed scanning optics set that enables video rate two-photon imaging. Our novel design allows the user to switch between either the 512x512 28 frames per second AOD mode or the conventional galvanometer-based mode 512x512 at 1 frame per second mode. Thus the user may select the image speed as required by the experiment.

Prairie View software controls all aspects of the scanner and collection of images while the optional VClamp software package is used to integrate electrical recording with line scan imaging, as well as Functional Mapping.
See example (data spotlight).

Integrated with a custom modified fixed stage microscope, the Ultima is ideal for studies involving the use of tissue slices or in-vivo work on small organisms such as mice.

features

Multi-channel analog integration electronics with 12 bit A/D

High Performance PC computer workstation with flat panel display

Prairie View scan control and image collection software

options

Uncaging galvanometer set

High Speed (28 fps) AOD scanning optics set

ExternalIV sub-stage detectors

Transmitted light gradient contrast detector

VClamp software