VClamp is the application that integrates electrophysiology recording with image collection. From VClamp, the user can define output signals for stimulation of a sample preparation or to control external equipment such as shutters or pumps. VClamp also allows the user to collect image and line scan data, and to use this information in the selection of discrete locations where photolysis is to be performed. In addition, this application allows the collection of Functional Maps, wherein the pixel intensity represents electrical activity recorded from an electrode while uncaging was performed at the pixel location. The resulting "map" therefore, provides a graphical representation of activity as a result of local uncaging.